Practical Guide for Biological Small-Angle Neutron Scattering Experiments and Data Analysis

Agenda

Biological Small-Angle Neutron Scattering Workshop

 

Teams link: See your Agenda that was emailed for the links.

Link to workshop website: https://conference.sns.gov/event/466/

 

Day 1: October 9, 2024

 

Schedule (EST)	Activity	Lead
9:30-9:45 am	Introductions	Hugh O’Neill
9:45-10:00 am	Center for Structural Molecular Biology Overview	Hugh O’Neill
10:00-10:30 am	Introduction to Small-Angle Neutron Scattering - Part 1	Volker Urban
10:30-11:00 am	Demonstration 1: Data reduction and background subtraction	Wellington Leite/ Sai Venkatesh Pingali/ Alan Hicks
11:00-11:15 am	Break
11:15-11:30 am	Virtual tour of Bio-SANS	Felicia Gilliland
11:30-11:45 am	Virtual Tour of Biology Laboratories	Qiu Zhang
11:44 am-12:45 pm	Lunch
12:45-1:15 pm	Introduction to Small-Angle Neutron Scattering -Part 2	Volker Urban
1:15-1:45 pm	Demonstration 2: Kratky plot, Guinier analysis, and Interparticle interferences	Wellington Leite/ Sai Venkatesh Pingali/ Alan Hicks
1:45 -2:00 pm	Break
2:00-2:30 pm	Demonstration 3: Molecular mass, Volume, and P(r) analysis	Wellington Leite/ Sai Venkatesh Pingali/ Alan Hicks
2:30-3:15 pm	Experiment Set-up and Data Acquisition	Sai Venkatesh Pingali
3:15-4:15 pm	Attendee Lightning Talks	Hugh O’Neill

 

 

Day 2: October 10, 2024

 

Schedule (EST)	Activity	Lead
9:30-11:00 am	Breakout and hands-on Session 1.0: Data analysis for solution scattering of biomacromolecular complexes Breakout and hands-on Session 2.0: Data analysis for hierarchical biosystems	Wellington Leite/ Sai Venkatesh Pingali/ Alan Hicks
11:00-11:15 am	Break
11:15 am-12:00 pm	Sample Preparation and Experiment Planning	Wellington Leite
12:00-1:00 pm	Lunch
1:00 - 1:20 am	Bio- and chemical deuteration	Kevin Weiss/ Honghai Zhang
1:20-2:50 pm	Breakout and hands-on Session 1.1: Data analysis for solution scattering of biomacromolecular complexes Breakout and hands-on Session 2.1: Data analysis for hierarchical biosystems (Free discussion)	Wellington Leite/ Sai Venkatesh Pingali/ Alan Hicks
2:50-3:00 pm	Break
3:00-3:30 pm	Q&A and Closing Remarks	Wellington Leite

 

Software required for Session 1

Software	Source	Application
ATSAS	EMBL	Program suite for small-angle scattering data analysis from biological macromolecules - https://www.embl-hamburg.de/biosaxs/software.html
RAW	BioXTAS	Program for analysis of Small-Angle X-ray Scattering (SAXS) data - https://bioxtas-raw.readthedocs.io/en/latest/api.html
Pymol	Schrödinger	Molecular visualization system - https://pymol.org/2/
Chimera	UCSF	Interactive visualization and analysis of molecular structures - https://www.cgl.ucsf.edu/chimera/

 

 

Software required for Session 2

Software	Source	Application
Irena	Igor Pro*	Irena package for analysis of small-angle scattering data https://usaxs.xray.aps.anl.gov/software/irena
SASView	SASView 5+	SASView for small-angle scattering analysis - https://www.sasview.org

 

A free demonstration version of Igor Pro is available at [https://www.wavemetrics.com/downloads/current/Igor%20Pro%209]]. This should be installed prior to installing the Irena package.