This workshop will provide hands-on training on applying and using Small-Angle Neutron Scattering (SANS) in structural biology. The workshop is an excellent opportunity for researchers who want to learn about different aspects of biological SANS, including how to perform SANS experiments using state-of-the-art capabilities at the SNS/HFIR and approaches to analyze SANS data. The workshop will cover lectures on the theoretical basis of small-angle scattering and practical aspects of sample preparation, data collection, and data analysis. There will be two sessions. The first will be an introduction of small-angle scattering concepts an experimental tutorial that will demonstrate how to plan an experiment, considerations to prepare samples, and performing SANS experiments at Bio-SANS. The second will focus on model and model-independent fitting and computational analysis of biological SANS data.
The workshop will cover the following topics:
Introduction to small-angle scattering.
Strategies and considerations for sample preparation, data collection, setup of data acquisition system “panel scan," and data reduction.
Data analysis topics covered will include model-independent analysis, contrast match point determination; fits for high-resolution validation; fit for single and multi-component protein complexes; and 3D reconstruction for low-resolution structure determination.